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The researchers identified these so-called epigenetic changes by using single nuclear ATAC sequencing (snATAC-seq) and single nuclear RNA-sequencing (snRNA-seq) to study RPE cells from young and aged mice three, six and 10 days post-cigarette smoke condensate (CSC) injection, and those exposed to cigarette smoke daily for four months

"Treatment" of sad emotional states with nicotine over time may also impair innate restorative responses, just as treatment of chronic pain with opioids results in many patients experiencing hyperalgesia, a pathologically heightened response to painful stimuli