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NNMT transfers a methyl group from S-adenosylmethionine (SAM) to nicotinamide (a form of vitamin B3), producing 1-methylnicotinamide

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The GraphPad Prism, version 8.4.3 (GraphPad Software, Inc., San Diego, CA, USA) was used to assess the significance of differences between two groups by Students t-test or a one-way ANOVA with a Bonferroni test

Finally, we found previous reports in support of an endogenous agonist mechanism for several class B GPCRs, including the secretin receptor and GLP-1R, in which short peptide motifs from the receptor ECD region can serve as receptor activator [50, 51]