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Importantly, participant age was not associated with BBB permeability in our cohort with long COVID, suggesting that BBB disruption is more probably due to the neurological symptoms of long COVID

Real Time qPCR was performed using SybrGreen Master Mix (ThermoScientific) with various sets of primers for specific genes

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Conclusions and future directions The lack of positive effects from CM supplementation within the existing literature is due to a number of factors, including the testing protocols not featuring a predominantly aerobic energy contribution, the lack of testretest reliability of exercise protocols, dosing strategy (i.e., amount and timing), and the recent discovery of quality control issues with some manufacturers stated citrulline:malate ratios