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10.1016/j.freeradbiomed.2015.06.019 Free Radic Biol Med

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Cell synchronization and flow cytometry analysis Cells were synchronized at the G1/early S-phase boundary using a double-thymidine (DT) block protocol: cells were cultured with 2 mM thymidine for 16 h, then released to thymidine-free medium for 8 h, and with 2 mM thymidine for another 16 h