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The research was supported by grants from the National Cancer Institute (CA106176, CA138833, CA095103, CA068485) and the National Institute of Diabetes and Digestive and Kidney Diseases (DK058404) of the National Institutes of Health.

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(3) our data suggest a model that elevated fat cell death upon IF attracts LAMs and/or induces the proliferation of LAMs in AT, worsening the inflammatory state of AT

Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter