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Reliable interpretation therefore requires biochemical context, rapid stabilization, and an analytical strategy matched to the biological question

All protocols included an overnight, approximately 18 h, exposure to the primary antibodies used and a two-hour exposure to fluorescently conjugated secondary antibodies

When ascorbic acid is oxidized, it forms semidehydroascorbate (1 degree of oxidation) and then dehydroascorbate (2 degrees of oxidation)

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