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Additionally, inconsistent findings regarding the role of Nrf2 at different stages of OA suggest that Nrf2 may have stage-dependent or even biphasic effects

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After washing the column material with a total of 250 mL of distilled water, a solution of Guanosine diphosphate (GDP, C 10 H 15 N 5 O 11 P 2 , 443.2 g/mol, 100 mg, 0.5 mmol, 1 equiv.) in distilled water (3 mL, c = 0.167 M) was applied to the column using a Pasteur pipette