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The protein pellet was resuspended in 100 L RIPA buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, and 140 mM NaCl) to normalize the intensity values for each targeted metabolite

Neither peptide shows significant acute toxicity at standard doses based on available evidence

(1) D Schneider-Helmert (1981) Effects of delta-sleep-inducing peptide on 24-hour sleep-wake behaviour in severe chronic insomnia European Neurology, 1987, Volume 27 (Issue 2), Pages 120-9

Proper injection technique, site rotation, and allowing alcohol to dry completely before injecting minimize these reactions