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It inhibits NOTCH1 signaling by using its GCT activity to cleave a -glutamyl-cysteine bond at glutamate 1669 of the immature NOTCH receptor, thereby preventing the essential S1 cleavage and subsequent activation ( FIGURE 6 The physiological importance of this mechanism is underscored by severe developmental phenotypes upon its disruption

Under normal conditions, NNMT activity can consume substantial amounts of both nicotinamide and SAM, particularly in metabolically active tissues or during periods of metabolic stress

The role of the immune system in osteoarthritis: mechanisms, challenges and future directions

PE/Cy7 anti-mouse CD4 antibody (BioLegend, 1:400)