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PEG grafting was probably limited by the effects of steric hindrance, and our results are in good agreement with Lee et al.s estimation of 25%50% PEG conjugation efficiency onto PVX, another high-aspect-ratio plant virus, in which there was estimated to be one grafted PEG for every three lysine residues ( Interestingly, the 5B formulation had 16% higher labeling than its PEG 2,000 counterpart ( Figure 2C ): 5B averaged 810 PEG per TMV vs

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ACTRN12613001207707 and NCT02328872), which is conducted and funded by the VCS Foundation

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