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If you reconstitute a multi-dose vial, picking a consistent day means you know exactly how long the vial has been reconstituted when you draw each dose

Fasting metformin concentrations were measured after the washout period at baseline (day 1, visit 2) and after the 3 month study (day 1, visit 5) ~12 h after the last study dose (ESM Table 1)

Nat Rev Gastroenterol Hepatol 15(7):397411